HPLC and HPLC-MS Analysis of Urinary N -Monomethyl-Lysine

HPLC analysis of GAGs in urinary stones

Glycosaminoglycans (GAGs) have been identified as one of the macromolecules present in the stone matrix. Although electrophoresis has been used to analyze the composition of GAGs, this method is laborious and insensitive. The aim of this study was to examine GAG profiles in the stone matrix from 4 types of stones using high-performance liquid chromatography (HPLC). This method has been shown to...

Differentiation of Cuscuta chinensis and Cuscuta australis by HPLC-DAD-MS analysis and HPLC-UV quantitation.

Cuscuta chinensis and Cuscuta australis, the two botanical sources of the Chinese herbal medicine Tu-Si-Zi, were distinguished from each other based on qualitative and quantitative chemical analysis. By HPLC‑DAD‑MS, a total of 36 compounds were characterized from these two Cuscuta species, including 14 flavonoids, 17 quinic acid derivatives, and 5 lignans. In addition, HPLC‑UV was applied to de...

Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS

BACKGROUND Xuesaitong (XST) injection, consisting of total saponins from Panax notoginseng, was widely used for the treatment of cardio- and cerebro-vascular diseases in China. This study develops a simple and global quality evaluation method for the quality control of XST. METHODS High performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to identify and quantify the chr...

HPLC-MS/MS of Highly Polar Compounds

HPLC/MS analysis of fusarium mycotoxins, fumonisins and deoxynivalenol.

Fusarium fungi are widely found in agricultural products, worldwide and can produce a great variety of mycotoxins. Fumonisins, produced by F. moniliforme, and deoxynivalenol, produced by F. graminearum, are two such mycotoxins that have received considerable attention as food safety concerns by regulatory agencies. High Performance Liquid Chromatography/Mass Spectrometry (HPLC/MS) was found to ...